https://bugs.winehq.org/show_bug.cgi?id=44960
--- Comment #6 from Harry Small 20917771@student.uwa.edu.au --- I am using Python 2.7 and pywin32 installed via PIP. Pyinstaller should have bundled the python interpreter for you, not sure why it did not work. The command is as you said: wine python.exe test.py