http://bugs.winehq.org/show_bug.cgi?id=9607
--- Comment #7 from Krzysztof Lichota krzysiek-bugs.winehq.org@lichota.net 2007-09-07 12:40:25 --- (In reply to comment #1)
Wine does not support running apps from a FAT partition.
Wine contains hooks which are valid only on FAT partition, like the mentioned VFAT_IOCTL_READDIR_BOTH ioctl.
I will re-test on wine 0.44, but from reading the source I am pretty sure it is the same.